The Split-Statistical Potentials Server (SPServer) is a tool designed to evaluate the energies of protein folds or protein-protein interactions based on the Split-Statistical Potentials.

The main result page displays the scores for each protein/interaction with available detailed data.

A detailed explanation on how to execute the server and interpret the results is found in Help:Server usage section. More information about Split-Statistical Potentials can be found in Help:About SPServer section, Help:FAQ section and on: Feliu E, Aloy P, Oliva B. On the analysis of protein–protein interactions via knowledge-based potentials for the prediction of protein–protein docking. Protein Science : A Publication of the Protein Society. 2011;20(3):529-541. doi:10.1002/pro.585.

  • Analysis set-up box: Select if you want to use the Split-Statistical Potentials to analise protein folds or protein-protein interactions (PPI). Then, choose the residue-residue contact definition (what is considered as a contact).
  • Input files(s) box: Fill in the inputs with the required data.
    • Protein folds: Upload a folder with all PDB to analise. The folder must be compresses in zip or tar.gz.
    • PPIs: Select if you yant to provide the PPI as a set of transforms to a ligand or as a set of PDB.
      • PDB structures (structures in separated files): Upload a folder with the PPI PDBs to analize, indicating wich one is the receptor (.r.pdb) and the ligand (.l.pdb). The folder must be compressed in zip or tar.gz.
      • PDB structures (structures in same file): Upload a folder with the PPI PDBs of the interactions to analize. Every PDB must only have two chains: one corresponding to the receptor, and the other to the ligand. The folder must be compressed in zip or tar.gz.

Analysis set-up



Input file(s)

IMPORTANT: Due to computational resoure limitations, the input files cannot exeed 10MB.
The file must contain protein structures in PDB format: .pdb extension. Any other files will be discarted
The file must be compressed in .tar.gz format.
Click here to use an example with precalculated data.
Click here to download an example of input data.
IMPORTANT: Due to computational resource limitations, the input files cannot exeed 10MB.


The file must be a protein structures in PDB format: .pdb extension. The file must be a protein structures in PDB format: .pdb extension. The file must be the original transformation file provided by the Docking program.
The file must contain pairs of protein structures in PDB format.
Every pair of structures must have the same name, but a suffix that indicates if it is receptor or ligand: .r.pdb or .l.pdb. Any other files will be discarted
The file must be compressed in .tar.gz format.
Click here to use an example with precalculated data.
Click here to download an example of input data.
The file must contain the protein structure of the interaction in PDB format and have only two chains. Any other files will be discarted
The file must be compressed in .tar.gz format.
Click here to use an example with precalculated data.
Click here to download an example of input data.